Watch: Humza Yousaf's resignation speech in full
Humza Yousaf said he made the decision to stand down as Scotland's first minister.
He said he made the decision after spending the weekend reflecting on what was best for the SNP, the government and the country.
Mr Yousaf added that someone else would need to take over in order to "repair our relationship across the political divide".
It follows the collapse of the SNP's power-sharing deal with the Greens.
Mr Yousaf's resignation comes just 13 months after Mr Yousaf won the contest to succeed Nicola Sturgeon as first minister and SNP leader after narrowly defeating Kate Forbes in what had been a fractious leadership race.
He became the first ethnic minority leader of a devolved government in the UK and the first Muslim to lead a major UK party.
